Clutterbuck, Abigail L. and Allaway, David and Harris, Pat and Mobasheri, Ali (2013) Curcumin reduces prostaglandin E2, matrix metalloproteinase-3 and proteoglycan release in the secretome of interleukin 1β-

Curcumin ┑diferuloylmethane┒ is a phytochemical with potent Objectiveジ antiゾinflammatory and antiゾoxidant propertiesゴ and has therapeutic potential for the treatment of a range of inflammatory diseasesゴ including osteoarthritis ┑OA┒ズ The aim of this study was to determine whether nonゾtoxic concentrations of curcumin can reduce interleukinゾ÷beta ┑ILゾ÷╅┒ゾstimulated inflammation and catabolism in an explant model of cartilage inflammationズ Articular cartilage explants and primary chondrocytes were obtained Methodsジ from equine metacarpophalangeal jointsズ Curcumin was added to monolayer cultured primary chondrocytes and cartilage explants in concentrations ranging from ≠]Mゾ÷××]Mズ Prostaglandin E ┑PGE ┒ and matrix metalloproteinase = = ┑MMP┒ゾ≠ release into the secretome of ILゾ÷╅ゾstimulated explants was measured using a competitive ELISA and western blotting respectivelyズ Proteoglycan ┑PG┒ release in the secretome was measured using the ÷ゴ∴ゾdimethylmethylene blue ┑DMMB┒ assayズ Cytotoxicity was assessed with a live┄dead assay in monolayer cultures after =< hoursゴ <∞ hours and five daysゴ and in explants after five daysズ Curcumin induced chondrocyte death in primary cultures ┑>×]M Resultsジ p4×ズ××÷ and ÷××]M ×ズ××÷┒ after =< hoursズ After <∞ hours and five daysゴ p8 curcumin ┑J=>]M┒ significantly increased cell death ┑ ×ズ××÷ both time points┒ズ p8 In explantsゴ curcumin toxicity was not observed at concentrations up to and including =>]M after five daysズ Curcumin ┑J≠]M┒ significantly reduced ILゾ÷╅ゾstimulated PG ┑ 4×ズ×>┒ and PGE release ┑ ×ズ××÷┒ from explantsゴ p = p8 whilst curcumin ┑J÷=]M┒ significantly reduced MMPゾ≠ release ┑ ×ズ×÷┒ズ p8 Nonゾcytotoxic concentrations of curcumin exert antiゾcatabolic and Conclusionジ antiゾinflammatory effects in cartilage explantsズ Referees v÷ published ×< Jul =×÷≠ v= published =× Aug =×÷≠ ÷ =